Browsing by Author "Kreft, Anna"

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    Addenum to the description of Steinernema jollieti Spiridonov, Krasomil-Osterfeld & Moens, 2004
    (The Russian Society of Nematologists, 2012-11-14) Skrzypek, Henryk; Kazimierczak, Waldemar; Kreft, Anna; Spiridonov, Sergei; Mráček, Zdeněk
    Additonal morphological data are provided for Steinernema jollieti Spiridonov, KrasomilOsterfeld, Moens, 2004. A light and scanning electron microscopy were used to particularize taxonomically important characters missing in the first decsription. The GS% and SW% indices are provided for males. The distribution of genital papillae on male tail is illustrated with SEM images. Structure of female tail and vulvar area are decsribed for females of the first and second generation. The relationships of S. jollieti with other steinernematid feltiae/kraussei group species are discussed.
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    Charakterystyka nicieni wyizolowanych z pędraków chrabąszcza Melolontha sp. (Coleoptera: Scarabaeidae)
    (Instytut Ochrony Roślin - Państwowy Instytut Badawczy, Polskie Towarzystwo Ochrony Roślin, 2012-06-15) Kreft, Anna; Kazimierczak, Waldemar; Sierpińska, Alicja; Skrzypek, Henryk; Skrabucha, Anna; Dybała, Magdalena; Dźwierzyńska, Monika
    Celem badań była charakterystyka nicieni porażających pędraki chrabąszcza Melolontha sp. w warunkach naturalnych. Pędraki chrabąszcza były zebrane na terenie nadleśnictw Krasnystaw, Kozienice, Opoczno i Brzeziny, na powierzchniach różniących się warunkami świetlnymi, wilgotnościowymi i temperaturowymi. Identyfikację gatunków przeprowadzono metodą sekwencjonowania fragmentów DNA (deoxyribonucleic acid) oraz metodami morfologicznymi przy zastosowaniu skaningowego mikroskopu elektronowego i mikroskopu optycznego z kontrastem interferencyjno-różnicowym. Uzyskane wyniki wykazały, że pędraki były porażone przez Diplogasteroides magnus oraz Koerneria sp. i Rhabditis sp. Większość zainfekowanych przez nicienie pędraków została zebrana na powierzchniach zadrzewionych, czyli tam, gdzie powierzchnia gleby była dobrze ocieniona, a wilgotność najwyższa.
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    Efektywność porażania larw chrabąszcza majowego (Melolontha melolontha L.) przez entomopatogeniczne nicienie
    (Instytut Ochrony Roślin - Państwowy Instytut Badawczy, Polskie Towarzystwo Ochrony Roślin, 2011-06-15) Kreft, Anna; Skrzypek, Henryk; Kazimierczak, Waldemar; Lech-Pituch, Ewelina; Skrabucha, Anna
    Experiments done and presented below aimed at proving that applied species and Polish strains of entomopathogenic nematodes (EPNs) can be used to reduce the number of Melolontha melolontha. Invasive larvae of Steinernema arenarium Bys, S. arenarium Lub, S. feltiae Rap, Heterorhabditis bacteriophora Sie, H. megidis Pla, H. megidis Lub i H. megidis Pla, H. megidis C3 were used in the experiments as biocontrol agents against the larvae L1, L2 and L3 of M. melolontha. The experiments were done at 20°C, in 25 ml boxes filled with pasteurized (3 h at 70°) and air-dried soil. Pathogen doses were 100 IJ/insect. The result showed that susceptibility of M. melolontha to EPNs depends on the insect's developmental stage. Larvae L1 were the most susceptible. S. arenarium Bys and H. megidis strains were more effective in infecting M. melolontha larvae than S. feltiae Rap and H. bacteriohora Sie.
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    Effectiveness of infection of Cameraria ohridella (Lepidoptera: Gracillariidae) larvae by entomopathogenic nematodes Steinernema sp. and Heterorhabditis sp. (Nematoda: Rhabditida)
    (Wydawnictwo UMCS, 2004-06-15) Kreft, Anna; Skrzypek, Henryk; Kazimierczak, Waldemar
    The purpose of the present studies was to evaluate the susceptibility of Cameraria ohridella larvae to infection by entomopathogenic nematodes Steinernema sp. and Heterorhabditis sp. in laboratory conditions. The experiments were done at the temperatures 20 ◦C and 25 ◦C with the use of three doses of the pathogen, namely 5, 25 and 50 nematodes/insect. The results showed that Cameraria ohridella larvae were susceptible to infection by entomopathogenic nematodes. Out of two strains of entomopathogenic nematodes used in the studies, the one that was more effective in infecting Cameraria ohridella at the temperature 20 ◦C was Steinernema sp., while at the temperature of 25 ◦C Heterorhabditis sp. infected the insects more intensively. Results of the experiments showed that with lower doses of invasive larvae of nematodes (5 larvae/insect, 25 larvae/insect) Steinernema sp. PIS81 was a more pathogenic strain towards Cameraria ohridella as compared to Heterorhabditis sp. PIH81.
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    Scanning electron microscopy study of infective juveniles Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae)
    (Wydawnictwo UMCS, 2004-06-15) Skrzypek, Henryk; Kazimierczak, Waldemar; Kreft, Anna
    Studies were conducted on the morphology of infective larvae Heterorhabditis bacteriophora using a scanning electron microscopy. The purpose of the studies was to point to the morphological and morphometric characters of infective larvae of entomogenous nematodes that are useful in taxonomy and species identification. It was found out that the studies using SEM make it possible to analyze the morphological characters unvisible in an optic microscope. Morphometric properties can also be examined in SEM, and since the measurements are very accurate they can be applied in taxonomic studies and in species identification. The preparation of the material for the studies in SEM and their performance is neither more labour-consuming nor more tiresome than in optic microscopy.