Wydział Medyczny / Faculty of Medicine

Permanent URI for this community

* Instytut Nauk Biologicznych * Instytut Nauk o Zdrowiu * Instytut Nauk Medycznych * Centrum Badań Eksperymentalnych

Now showing 1 - 1 of 1

The Anti-Candida albicans Agent 4-AN Inhibits Multiple Protein Kinases
(MDPI, 2019) Masłyk, Maciej; Janeczko, Monika; Martyna, Aleksandra; Czernik, Sławomir; Tokarska-Rodak, Małgorzata; Chwedczuk, Marta; Foll-Josselin, Béatrice; Ruchaud, Sandrine; Bach, Stéphane; Demchuk, Oleg M.; Kubiński, Konrad
Small molecules containing quinone and/or oxime moieties have been found as promising anti-fungal agents. One of them is 4-AN, a recently reported potent anti-Candida compound, which inhibits the formation of hyphae, decreases the level of cellular phosphoproteome, and finally shows no toxicity towards human erythrocytes and zebrafish embryos. Here, further research on 4-AN is presented. The results revealed that the compound: (i) Kills Candida clinical isolates, including these with developed antibiotic resistance, (ii) affects mature biofilm, and (iii) moderately disrupts membrane permeability. Atomic force microscopy studies revealed a slight influence of 4-AN on the cell surface architecture. 4-AN was also shown to inhibit multiple various protein kinases, a characteristic shared by most of the ATP-competitive inhibitors. The presented compound can be used in novel strategies in the fight against candidiasis, and reversible protein phosphorylation should be taken into consideration as a target in designing these strategies.